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. 2017 Jul 7;6(8):1684–1697. doi: 10.1002/sctm.17-0078

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© 2017 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Expression of pancreatic islet β cell‐related markers in platelets. (A): Real time PCR analysis of pancreatic islet‐related hormone products and β‐cell‐related functional markers in CB‐platelets (n = 6). Freshly isolated human islets served as positive controls. (B): Real time PCR analysis of pancreatic islet β‐cell‐related transcription factors in CB‐platelets (n = 6). (C): Western blotting shows the protein expression of an islet β cell‐specific transcription factor MAFA in CB‐platelets. (D): Flow cytometry for human pancreatic islet‐related hormone products in freshly‐isolated human pancreatic islet cells. (E): Flow cytometry for the pancreatic islet‐related hormone products by double staining with platelet markers CD41 or CD42 in CB‐platelets. (F): Flow cytometry for pancreatic islet β‐cell‐related transcription factors by double staining with platelet markers CD41 or CD42 in CB‐platelets (n = 7). (G): Real time PCR analysis of pancreatic islet‐related hormone products and β‐cell‐related functional markers in PB‐platelets (n = 6). (H): Real time PCR analysis of pancreatic islet β‐cell‐related transcription factors in PB‐platelets (n = 6). (I): Flow cytometry for pancreatic islet‐related hormone products by double staining with platelet markers CD41 or CD42 in PB‐platelets (n = 15). (J): Flow cytometry of pancreatic islet β‐cell‐related transcription factors in PB‐platelets (n = 8). (K): Confocal microscopy of human CB‐ and PB‐platelets after triple immunostainings with insulin (blue), dense granule marker ADP (red), and α granule marker vWF (green). Isotype‐matched IgGs served as controls (inserted yellow dashed rectangle). Scale bars, 5 μm. Representative data were from six experiments.