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Protein size and heterogeneity analysis of dmLT. (a) SDS-PAGE analysis of dmLT under nonreducing (−DTT) and reducing conditions (+DTT), (b) SV-AUC studies of dmLT. Sedimentation coefficient distribution are shown from 0 to 14 svedbergs. The inset shows the distributions from 6 to 14 svedbergs to better visualize the aggregate peaks, (c) representative HIC chromatogram of dmLT sample, and (d) representative RP-UHPLC chromatogram of dmLT sample. DTT, dithiothreitol.
