Figure 2.

Gab2 ablation suppresses the development of DEN-induced primary liver cancer in mice. A) Characteristics of livers from mice that were treated with DEN for 12 mo. Arrows indicate liver cancer nodules. B–E) Tumor count per animal (B), average maximum tumor diameter (C), average tumor size (D), and average liver-to-body weight ratio (E) were compared between WT-saline, WT-DEN, Gab2-KO-saline, and Gab2-KO-DEN mice. F) Gab2 expressions of 4 group mouse models were shown with IHC staining. G, I) Representative IHC images of Ki-67 expression (G) and the number of Ki-67–positive cells (I) in liver tissue from DEN-treated mice [n = 5 high-power fields (HPFs) for each mouse]. H, J) pH3 expression by IHC (H) and statistical analysis of pH3-positive cells (J) in 4 groups of mice. Brown color indicates positive staining (n = 5 HPFs for each mouse). *P < 0.05, **P < 0.01.