Figure 2.

Plasma metabolites, body weight, and weight gain as markers of successful rescue of KO mice with PEG-CBS treatment. A) Plasma concentrations of Hcy, Cth, Cys, and Met after weekend washout (trough, solid bars) and 24 h after the last injection (peak, hatched bars) of 40MA (blue) or 20NHS (red) PEG-CBS compared with PBS-injected heterozygous siblings (black). B, C) Body weight (B) and weight gain (C) of 20NHS PEG-CBS–treated CBS-KO male (red solid line and circles) and female (red dashed line and circles) mice compared with PBS-injected heterozygous male (black solid line and squares) and female mice (black dashed line and squares). The number of subjects in each group is indicated in the plots. Mice received subcutaneous injections 3 times a week of 7.5 mg/kg from d 2 of age until d 35. *P < 0.05, ***P < 0.001.