Figure 6.

Long-term treatment of the KO mice with 20NHS PEG-CBS sustains an improved plasma metabolite profile. The KO mice (n = 5 M + 7 F) were treated 3 times a week with 20NHS PEG-CBS (SC, 7.5 mg/kg) from d 2 of age for ∼5 mo. The initial levels at d 42 do not represent true basal concentrations of the metabolites prior to the treatment initiation because KO mice were treated from birth to prevent neonatal lethality. Instead, the initial levels correspond to plasma concentration of the metabolites 72 h after the injection (i.e., after a weekend washout), when mice were old enough to survive blood collection. Relevant sulfur amino acids are shown: Hcy (solid line with squares), Cth (dashed line with circles), and Cys (dotted line with diamonds). Symbols represent an average value from individual mice. Error bars indicate sem.