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. 2017 Jul 31;31(12):5172–5183. doi: 10.1096/fj.201700211R

Figure 2.

Figure 2.

Attenuated Ca2+ mobilization in human endothelial cells cultured in various levels of O2. HUVECs were maintained at 18% O2 or cultured in 5 or 1% O2 for at least 5 d. Cells were stimulated with histamine (10 µM), and [Ca2+]i was monitored. A) Ca2+ transients in HUVECs stimulated with histamine (10 µM) in 18, 5, or 1% O2. B) Average peak and plateau [Ca2+]i values in response to histamine. C) Plateau [Ca2+]i levels in response to ATP (10 µM) or ionomycin (0.1 µM). D) HUVECs were loaded with Cal520-AM and then exposed to 15 dyn/cm2 unidirectional shear stress for 10 min, followed by an acute stimulation with histamine. Representative images are provided from HUVECs at 18% O2. E) Calmodulin (CaM) expression in HUVECs in 18, 5, or 1% O2. Representative immunoblots and analysis of CaM expression relative to β-actin. Treatment with histamine was significant (P < 0.05 vs. respective control) in relevant panels. Data are means ± sem (n = 4–11 different donors). **P < 0.01, ***P < 0.001 vs. 18% O2; #P < 0.05 vs. 5% O2.