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. 2017 Jul 31;31(12):5172–5183. doi: 10.1096/fj.201700211R

Figure 6.

Figure 6.

Impact of O2 on NO bioavailability in endothelial cells. A) Modeling of NO release from different levels of DETA after 10 min of decomposition. B) HUVECs were maintained at 18% O2 or cultured in 5% O2 for at least 5 d. Cells were treated with DETA (0–500 µM, 10 min), and cGMP production was assayed. Vehicle (NaOH 0.05%) had no effect on cGMP production (not shown). C) Correlation of predicted [NO] with measured cGMP levels. Differences in neither slope nor intercept were significant when 18 and 5% O2 were fitted separately, so one equation represents both O2 levels. D) Histamine (10 µM, 5 min) stimulated cGMP levels in HUVECs cultured in 18% O2 and then exposed acutely to 5% O2 for 30 min. Treatment with histamine was significant (P < 0.05 vs. respective control). Data are means ± sem (n = 3–9 different donors). **P < 0.01, ***P < 0.001 vs. 18% O2; ##P < 0.01 vs. 5% O2 (5 d).