Fig 2. Optimized siRNA screening condition.

(A) Huh7 cells were seeded into a 384-well plate with 1000, 2000, 3000, 4000, and 5000 cells per well. Cell morphology and confluency were observed and an image was captured 72 hours later using a phase contrast microscopy. The arrow indicates the optimized seeding condition for siRNA screening assay. (B) Huh7 cells were reverse transfected with a siNTC or siCaspase 3 for 24 hours before infected with DENV at MOI of 10 for 48 hpi. Conditions were replicated in 15 wells within the same plate. Caspase 3 activity was measured and plotted across the 15 wells.