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. 2017 Nov 16;12(11):e0188121. doi: 10.1371/journal.pone.0188121

Fig 3. Knockdown efficiency of TNFSF12 and SPHK in DENV-infected Huh7 cells.

Fig 3

Huh7 cells were reverse transfected with siRNA targeted TNFSF12 or SPHK2 genes or the non-targeting control siRNA for 24 hours before infected with DENV for 48 hours. The mRNA expression was then analyzed using real-time RT-PCR. The mRNA expression of TNFSF12 (A) and SPHK2 (B) represented as fold times compared to parental control. The results are expressed as the average of three independent experiments ± SD. The asterisks indicate statistically significant differences between groups (p < 0.05) (Student’s t test).