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. 2017 Nov 16;12(11):e0187978. doi: 10.1371/journal.pone.0187978

Fig 1. Effects of NTP treatment on cell migration, cell death, cell viability and protein expression in KFs and NFs.

Fig 1

(A) Scratch wound healing assay after NTP treatment on NF and KF. Wound healing was documented by photography. (B) Annexin-V and propidium iodide staining in NFs and KFs. (C) MTT assay for cell viability of NFs and KFs. (D) Western blotting analysis for T-EGFR, Type I collagen, p-STAT3, T-STAT3, p-AKT, T-AKT, p-FAK, T-FAK, vimentin, p-ERK, T-ERK in NFs and KFs after NTP treatment. (E)Immunofluorescent staining of NFs and KFs was performed using p-STAT3 and F-actin antibody. DAPI was used for nuclei counter staining. After NTP exposures for 10, 30, or 60 s and after the control gas treatment.(Green = p-STAT3, RED = phalloidin, F-actin, Blue = DAPI). Data represent mean±standard deviation of three independent experiments. Each figure was representative of three experiments with triplicates. Scale bar = 100 μm. *P < .05, **P < .01 and ***P < .001.