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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Bone. 2017 Sep 21;105:245–252. doi: 10.1016/j.bone.2017.09.012

Fig. 2. 3D-networked osteocyte construction using primary human bone cells.

Fig. 2

(a) hip fragment shown as an example; (b) as-isolated cells after 4 collagenase digestion cycles; (c) proliferated osteoblastic cells after 10 days of 2D culture; (d) 3D tissue sample constructed using 20–25 µm microbeads and proliferated cells and 14 days of perfusion culture; (e) H&E histologic images showing the formation of 3D cellular network as indicated by black arrows in (f) and white arrows in (g); and (h) immunostaining for sclerostin (red). (d) –(f) from patient sample #6 and (g)–(h) from patient sample #4. Scale bar: 25 µm.