Fig. 4.

Co-targeting Ral-Fum hybrids have anti-ER activity in breast cancer cells. a MCF-7 cells were transfected with ERE and renilla reporter plasmids. Cells were then treated with various concentrations of drugs for 1 h, followed by E2 (10 nM) for 4 h to activate ER. Each drug’s inhibitory activity was calculated as percentage of E2 alone, which is set to 100%. b MCF-7 cells were treated with various concentrations of drugs for 1 h, followed by E2 (10 nM) for 2 h. mRNA expression of PR was measured by RT-QPCR. Each drug’s inhibitory activity was calculated as percentage of E2 alone, which is set to 100%