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. 2017 Nov 16;12(11):e0188064. doi: 10.1371/journal.pone.0188064

Fig 4. Control and seizure animals express different ratios of type II and type I BK channels.

Fig 4

(A-B) Type II channels have longer open dwell times and were resistant to IBTX. Type I channels had observably shorter open dwell times and were consistently blocked by IBTX. All channels were blocked by paxilline. (C) β4 KO mice only express Type I channels that are sensitive to IBTX and paxilline. (D) Type I BK channels were more predominant from seizure experienced mice (Control n = 15, Seizure n = 19). (E) Single channel current amplitude was similar in control, pilocarpine treated, and β4 KO mice. Single channel current was an average of 7 ± 1.5 pA at a 0 mV holding potential. (Control n = 8, Seizure n = 7, β4 KO n = 5) (F) The average number of BK channels per membrane patch was similar in control and seizure mice. (Control n = 15, Seizure n = 19). Panels A-E data was acquired at 0 mV potential with 60 μM buffered calcium internal solution.