Fig 5. Seizure activity switches predominant BK channel gating properties from type II to type I.
(A) The average open dwell time of BK channels recorded from pilocarpine treated mice was more similar to β4 KO channels than they were to control recordings. (B) The seizure driven loss of β4 reduces the NPO of channels at a -40 mV holding potential. (Control n = 8, Seizure n = 7, β4 KO n = 5) (C) Independent of seizure history, the dwell times of IBTX sensitive (and IBTX resistant) BK channels were similar. (Control IBTX sensitive n = 4, Control IBTX resistant n = 6, Seizure IBTX sensitive n = 5, Seizure IBTX resistant n = 4). Recordings were made with 60 μM buffered calcium internal solution.