Table 1. Spawning performance of females whose eggs were subjected to proteomic analyses.

Experiment	Egg quality type	Female number	Eggs total(N)	Eggs incubated	% Intact eggs 2–3 hps	% Survival 8 hps	% Survival 24 hps	% Survival48 hps	% Survival 72 hps
Pooled Samples	Good	1	115	60	100	100 (1.7)	98.3 (0)	98.3 (0)	98.3 (0)
		2	216	60	100	100 (0)	100 (0)	100 (0)	100 (0)
		3	355	60	100	100 (0)	100 (0)	100 (0)	100 (0)
		4	401	60	100	100 (0)	100 (0)	100 (0)	100 (0)
	Poor	5	129	60	96.7*	91.7 (96.4)	3.3 (0)	3.3 (0)	3.3 (0)
		6	224	60	100*	0	0	0	0
		7	165	53	100*	79.2 (76.2)	11.3 (0)	11.3 (0)	11.3 (0)
		8	266	63	100*	17.5 (0)	14.3 (0)	14.3 (0)	14.3 (0)
Multiple Samples	Good	9	401	60	100	100 (0)	100 (0)	100 (0)	100 (0)
		10	355	60	100	100 (0)	100 (0)	100 (0)	100 (0)
		11	216	60	100	100 (0)	100 (0)	100 (0)	100 (0)
		12	124	60	100	100 (0)	100 (0)	100 (0)	100 (0)
	Poor	13	251	60	100*	0	0	0	0
		14	187	60	100*	13.3 (0)	11.7 (0)	11.7 (0)	11.7 (0)
		15	481	60	100*	6.7 (0)	6.7 (0)	6.7 (0)	6.7 (0)
		16	404	61	96.7*	85.2 (67.3)	16.4 (0)	16.4 (0)	16.4 (0)

Shown are the experiment, egg quality type, female number, total number of eggs spawned, number of eggs incubated, percentage (%) of eggs intact at 2–3 hours post spawning (hps), and percentage of eggs yielding embryos surviving to 8, 24, 48 and 72 hps with the percentage of these that were abnormal shown in parentheses. For intact eggs at 2–3 hps, asterisks indicate that a high proportion of abnormal embryos showing asymmetric cell cleavage and/or early developmental arrest were observed but not quantified. The percentage of eggs yielding normal embryos surviving to 24 hps (bold text on gray background) was used to assign females to egg quality groups.