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. 2017 Nov 16;12(11):e0186769. doi: 10.1371/journal.pone.0186769

Fig 2. Assessment of unfolded protein response markers: Phosphorylated inositol-requiring kinase 1 (IRE1) and binding immunoglobulin protein/78-kDa glucose-regulated protein (BiP/GRP78).

Fig 2

Double immunofluorescent staining of cross-sections of kidneys using anti-phospho-IRE1 (S724) and anti-THP (left column) or using anti-BiP/GRP78 (right column) and anti-THP-(merged double-staining images in both columns; yellow color denotes colocalization) showed that both phosphorylated IRE1and BiP/GRP78 were absent in tubular-cells (TAL cells) that were strongly labeled with anti-THP antibody in 1-month (1M) old WT mice (the first row). In contrast, phosphorylated IRE1 and BiP/GRP78 were highly expressed in the THP-positive TAL cells in age-matched THP-C217G mutant mice (the second and third rows). Insets (solid white line-boxes) showed higher magnifications of the areas in dash-lined boxes. However, phosphorylated IRE1 and BiP/GRP78 were undetectable in 12-month old (12M) WT mice (the fourth row) as well as in THP-C217G mutant mice (the fifth row). Nuclei were labeled blue by DAPI. Magnification for all panels, 400x and insets, 800x.