Table 1. Human forward (F) and reverse (R) primers used for real-time PCR.

Gene	Accession No.	Primer sequence	Expected size (bp)
COL2a1	XM_017018831.1	(F) 5’-CAACAACCAGATTGAGAGCA-3’	166
		(R) 5’-CCATGTTGCAGAAAACCTTC-3’
ACAN	NM_013227.3	(F) 5’-GGAGCAGGAGTTTGTCAACA-3’	186
		(R) 5’-CTTCTCGTGCCAGATCATCA-3’
CCN2	NM_001901.2	(F) 5’-GCAGGCTAGAGAAGCAGACC-3’	152
		(R) 5’-ATGTCTTCATGCTGGTGCAG-3’
5-HT2AR	NM_001165947.2	(F) 5’-TGGATCGGTTATCTCTCTTC-3’	149
		(R) 5’-AGCCGGTATTGTGTTCACTA-3’
5-HT2BR	NM_001320758.1	(F) 5’-GCACTGGGCAGCTCTTCTGA-3’	149
		(R) 5’-CCAACCAGCAAATCAGCCAC-3’
5-HT2CR	NM_001256760.2	(F) 5’-TTTCAATTCGCGGACTAAGG-3’	96
		(R) 5’-GTCCCTCAGTCCAATCACAG-3’
TpH-1	NM_004179.2	(F) 5’-TGCAAAGGAGAAGATGAGAGAATTTAC-3’	113
		(R) 5’-CTGGTTATGCTCTTGGTGTCTTTC-3’
GAPDH	NM_001289746.1	(F) 5’-GCCAAAAGGGTCATCATCTC-3’	214
		(R) 5’-GTCTTCTGGGTGGCAGTGAT-3’