Fig 3. Morphological lung changes after LPS treatment.

(A) Representative images of hematoxylin and eosin stained lung sections of five animals from each experimental group. Lungs were fixed with 4% paraformaldehyde, embedded in paraffin, and cut into 5 μm thick sections before being stained. Photomicrographs were obtained with a Nikon Eclipse E800 microscope. Both WT and sEng+ mice lungs show marked inflammatory infiltrates (arrow) after LPS treatment, inter-alveolar septal thickening (arrow head), and interstitial edema (•). Magnification x200 and x400. (B) Severity of lung injury was scored by a pathologist using a semiquantitative histopathology score system which evaluates lung injury in four categories: alveolar septae, alveolar hemorrhage, intra-alveolar fibrin, and intra-alveolar infiltrates. Data are expressed as mean ± SEM. n = 5 in each group of mice, +p<0,0001 vs control, two-way ANOVA. (C) Evaluation score of intra-alveolar infiltrates. n = 5 in each group of mice.