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. 2017 Nov 16;12(11):e0188413. doi: 10.1371/journal.pone.0188413

Fig 2. Immunohistochemical classification of neural fibers in mouse dorsal prostate.

Fig 2

(A) Paraffin embedded adult mouse dorsal prostate sections (5 μm thickness) were stained with DAPI and antibodies against (B) MBP and TUBB3, (C) TH and TUBB3, CGRP and TUBB3, or (E) SLC18A3 and TUBB3. Identified cells include (b1) MBP1+;TUBB3- Schwann cells, (c1) TH+;TUBB3+ adrenergic fibers, (d1) CGRP+;TUBB3+ sensory fibers, and (e1) SLC18A3+;TUBB3+ cholinergic fibers. Images are representative of three mice. Abbreviations are: MBP, myelin basic protein; CGRP, calcitonin-gene-related peptide; SLC18A3, solute carrier family 18 member 3; TH, tyrosine hydroxylase; DAPI, 2-(4-amidinophenyl)-1H -indole-6-carboxamidine; Scale bar is 25 μm.