Fig 7. Immunohistochemical characterization of Wnt10a^CreERT lineage.

Wnt10a^CreER/+T;tdtomato^fl/fl and Wnt10a^+/-;tdtomato^fl/fl (control) male mice were given a single intraperitoneal injection of tamoxifen (100 mg/kg) on postnatal day 3 and aged to two months. Prostates were sectioned (5 μm thickness), and stained with DAPI and antibodies against (B) KRT5,SYP;KRT8/18, and RFP/tdtomato. The tdtomato lineage label was identified in KRT5+;SYP-;KRT8/18- basal epithelial cells and KRT5-;SYP-;KRT8/18+ luminal epithelial cells in the (A) dorsal prostate (B) prostatic urethra of cre expressing mice but (C-D) not in the same regions of no cre control mice. Images are representative of three mice. Abbreviations: SYP, synaptophysin; KRT5, keratin 5; KRT8/18, keratin 8/18; RFP, red fluorescent protein; DAPI, 2-(4-amidinophenyl)-1H -indole-6-carboxamidine; Scale bar is 25 μm.