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. Author manuscript; available in PMC: 2018 Nov 15.
Published in final edited form as: Clin Cancer Res. 2017 Aug 30;23(22):6833–6845. doi: 10.1158/1078-0432.CCR-17-1087

Table 3a.

Development of brachyury-specific T-cell responses by intracellular cytokine staining

Carcinoma
Clinical characteristics Cycle I Cycle II Cycle III Late Any post
Dose level CD4 CD8 CD4 CD8 CD4 CD8 CD4 CD8
Pt Cancer CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF
2 Ovarian 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 17 0 0/1 (0-%)
15 Breast 2 0 0 6 0 0 471 0 894 2121 0 0 194 0 0 0 458 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7/10 (70%)
18 Breast 2 0 0 70 0 0 0 79 0 0 0 75 0 0 0 0 0 0 0 290 0 0 0 32 0 0 0 98 0 0 0 0 0
25 Breast 2 603 948 0 355 0 395 0 217 1771 134 0 225 0 268 67 382 0 835 3480 767 0 0 29 104
7 Colon 2 0 8 0 0 0 0 96 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
17 Colon 2 0 0 0 389 0 0 0 446 0 0 0 0 0 191 0 0 87 0 0 0 0 0 0 0 285 0 44 0 0 135 0 45
23 Colon 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 56 0 0 0 0 0 0 0 0
24 Colon 2 0 0 0 0 0 366 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
11 Lung 2 1228 4524 1120 1300 71107 15652 45 8323 0 0 0 0 0 0 0 0 0 217 0 46 0 0 0 0 0 0 0 0 0 0 0 0
6 Pancreatic 2 0 0 0 0 0 0 0 0 0 54 0 0 0 0 0 0
22 Prostate 2 0 0 0 0 0 0 0 0 0 0 0 0 3400 654 0 2558 0 0 2557 0 0 0 0 0
27 Breast 3 0 0 0 136 0 0 0 0 0 256 0 38 0 0 0 0 0 0 121 0 0 0 108 0 11/12 (92%)
38 Breast 3 0 0 0 0 0 0 0 0 0 0 0 5581 0 735 0 8763 39149 23008 5240 46867 19438 2608 0 10472
14 Colon 3 19 0 128 0 1858 335 0 633 0 0 102 0 3762 391 0 1286 0 0 0 0 0 0 0 0 0 388 140 0 0 289 0 0
26 Colon 3 0 0 0 0 0 0 162 0 304 2150 0 2500 3086 96 0 1259 0 929 0 973 0 223 0 0
32 Colon 3 0 516 0 51 0 23 0 0 0 0 0 0 0 188 0 0 169 11 0 687 0 0 0 0
33 Colon 3 463 0 146 0 0 0 0 0 0 0 0 0 0 0 0 0 2461 196 0 0 6971 0 0 0
28 Lung 3 0 0 0 0 0 0 0 0 0 37 0 0 0 122 0 235 857 265 0 0 4417 507 0 788
34 Lung 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
36 Lung 3 1039 250 1551 1825 2894 411 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 182 0 69
12 Pancreatic 3 404 0 74 0 0 0 0 0 271 348 0 479 1982 0 10 148 0 0 0 0 0 0 0 0
19 Prostate 3 0 0 0 0 0 0 0 0 0 0 394 0 0 0 0 0 0 0 1686 0 0 0 301 0
29 Prostate 3 0 0 21 0 0 0 0 0 0 0 128 0 1116 0 0 0 607 0 58 0 1276 0 0 0
Chordoma
Clinical characteristics Cycle I Cycle II Cycle III Late Any post
Dose level CD4 CD8 CD4 CD8 CD4 CD8 CD4 CD8
Pt Cancer CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF CD107a IFNg IL2 TNF
1 Chordoma 1 0 0 415 0 2125 0 0 718 0 0 0 0 0 177 0 0 0 0 0 0 0 0 5 129 2/2 (100%)
3 Chordoma 1 0 308 0 112 0 0 34 0 0 0 0 0 0 0 0 0 0 0 0 83 0 0 49 0 43 0 0 30 0 0 0 0
4 Chordoma 2 280 0 0 695 0 0 0 0 0 0 0 0 1592 207 0 804 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 535 5/5 (100%)
5 Chordoma 2 0 0 0 0 0 0 0 0 838 193 38 112 1148 0 0 442 0 0 0 0 0 0 6 0 0 0 0 30 0 11 73 0
8 Chordoma 2 216 0 0 0 0 749 0 1640 0 0 0 0 0 120 0 120 382 0 0 0 5278 239 0 302
9 Chordoma 2 0 0 0 0 0 253 0 0 0 0 268 0 0 173 0 0 0 0 206 0 0 46 0 0
10 Chordoma 2 538 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 488 0 0 0 3955 0 24 0 0 0 0 0 0 0 0 0
16 Chordoma 3 0 0 36 0 1309 0 0 0 0 248 546 35 6327 2079 141 0 1324 677 0 323 5761 0 0 0 0 159 0 126 0 0 37 0 3/4 (75%)
21 Chordoma 3 0 0 0 56 0 0 0 0 0 1640 240 1566 9455 57 307 1554
30 Chordoma 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 96 0 0 0 0
37 Chordoma 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1629 0 0 280 552 0 0 0 3655

Absolute number of CD4 of CD8 T cells producing cytokine or positive for CD107a per 1×106 cells plated at start of IVS.

Numbers in bold and great are those positive after versus before vaccination.

Gray indicates a patient meeting defined immune response criteria.