Table 1.
Treatment with CFI-402257 causes premature progression through the mitotic division, resulting in accumulation of mitotic aberrations in MM cells.
| Cell line | Condition | cells in meta-/ana-phase (%) | cells in telophase (%) | division errors (%) |
|---|---|---|---|---|
| Phi | Vehicle | 10.85 ± 1.65 | 0.43 ± 0.35 | 8.00 |
| CFI-402257 | 4.89 ± 1.55 | 9.92 ± 2.29 | 34.23 | |
| Hmeso | Vehicle | 10.83 ± 2.99 | 0.33 ± 0.62 | 10.35 |
| CFI-402257 | 4.28 ± 1.58 | 8.32 ± 2.39 | 43.36 |
Phi and Hmeso cells (105 cells/slide chamber) were pretreated with 50 ng/mL nocodazole for 10 hr to induce G2/M arrest and then released in full media with CFI-402257 or DMSO for 30 min. Cells were stained for mitotic spindle (α-tubulin) and nuclei (DAPI). First, cells in metaphase/early anaphase and telophase were counted. A total of 1400 cells (Phi) and 800 cells (Hmeso) were examined per treatment condition. Next, microscopic analysis of dividing cells was performed and cells exhibiting errors such as lagging chromosomes and unequal division were counted out of 50 cells in telophase. Two independent experiments were performed. Mean ± SD were calculated with Student’s t test.