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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Connect Tissue Res. 2017 Jun 8;59(2):191–200. doi: 10.1080/03008207.2017.1330333

Table 1.

Primer sequence for real-time PCR.

Name Length Tm Sequences Gene ID
mmp13 forward 175 57.6 5′-CCTTCTGGTCTTCTGGCTCAC-3′ NM_001082037.1
mmp13 reverse 5′-AGTGCTCCTGGGTCCTTGG-3′
mmp1 forward 199 55.2 5′-TGGAGTGCCTGATGTG-3′ NM_001171139.1
mmp1 reverse 5′-CTTGACCCTTGGAGACTTTGG-3′
collagen-II forward 60 55 5′-ACAGCAGGTTCACCTATAC −3′ XM_002723439.3
collagen-II reverse 5′-CCCACTTACCGGTGTT −3′
aggrecan forward 176 60 5′-AGGATGGCTTCCACCAGTGC −3′ XM_008251723.2
aggrecan reverse 5′-TGCGTAAAAGACCTCACCCTCC-3′
interleukin-6 forward 148 53.1 5′-CGGCGGTGAATAATGAGAC −3′ NM_001082064.2
interleukin-6 reverse 5′-ATCAGGTGTTGGATGTTCTTC −3′
cox-2 forward 106 55 5′-AACTCCCAATCCGCATGCTA −3′ NP_007552.1
cox-2 reverse 5′-GCTTGATTTAAGCGTCCGGG −3′
18s forward 167 55 5′-ATCAGATACCGTCGTAGTTC −3′ NR_033238.1
18s reverse 5″-TTCCGTCAATTCCTTTAAG-3″