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. 2017 Nov 16;8:1568. doi: 10.1038/s41467-017-01924-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Phylogram representation of B-cell responses to Pfs25-VLP immunization. Natively paired H- and L-chain, full-length, variable region sequences from 13 Pfs25-VLP-immunized Kymice were combined into one phylogram based on full-variable region H- and L-chain amino acid sequence similarity among plasmablast IgG and memory B-cell IgG sequences. The 95 plasmablast-derived and 18 memory B-cell-derived sequences selected for recombinant antibody expression are noted as green circles and blue boxes, respectively. Recombinant antibodies listed in Table 1 chosen for further analysis are labeled. Between pairs of nodes, sum of radial distances is proportional to the Kimura distance as indicated by the scale bar. Branch color denotes the specific animal from which the sequence was derived. Higher magnification insets show cases in which two different anti-Pfs25 antibodies of highly similar sequence originate from different individual Kymice and, in two cases, different antibody-expressing cell types. The subset of plasmablast sequences from Pfs25-VLP immunized Kymice that are similar to sequences from empty VLP-immunized Kymice are included as part of the 1564 plasmablast data set shown in the tree, but were not used as sources for recombinant antibody expression