Fig. 5.

Environmental regulation of RNA editing in the NFS1 and SFS populations. a Scatterplots comparing average biological replicate 1 and replicate 2 mmPCR-seq editing levels for NFS1 and SFS samples at 25 and 18 °C. Gray points represent sites with >5% editing level differences between replicates. b Scatterplots comparing the average editing levels of NFS1 and SFS flies between 18 and 25 °C. Red dots represent sites with >5% editing level difference between the temperatures, and FDR-adjusted p-value <0.05 (t-test). The number of fly lines represented for each of these sites per population ranges from 3 to 8 for NFS1 and 3 to 16 for SFS; Supplementary Data 8. c For NFS1 and SFS flies, plots comparing predicted RNA structure free energy levels for sites that show <2% editing level difference between 18 and 25 °C (black), and sites that show >5% editing at 18 °C than 25 °C (red) (p-value = 0.0393 for NFS1 lines, p-value = 0.0949 for SFS lines, one-sided KS-test). d For differentially edited mmPCR-seq sites shown in Fig. 3a that had adequate coverage for samples at 18 °C (see Methods section for details), compare average editing level differences at 18 °C (turquoise) and 25 °C (red). Error bars represent standard deviation. The number of fly lines represented for each of these sites per population ranges from 4 to 8 for NFS1 and 10 to 16 for SFS; Supplementary Data 9. e Plot showing the editing levels of an editing site in falafel that shows an interaction between genetics and environment (FDR-adjusted p-value = 0.065, ANOVA test). Editing levels at 18 °C are shown in turquoise and at 25 °C are shown in red. Error bars represent standard error of the mean. Editing levels for the SFS fly population are represented by all 16 SFS lines, while those for the NFS1 population are represented by 7 and 8 NFS1 lines for 18 and 25 °C, respectively