Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 17;8:1591. doi: 10.1038/s41467-017-01661-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — tpCD4⁺ T cells-mediated antigen presentation generates CD8⁺ T central memory. a, b In vivo proliferation of CellTrace Violet-stained OT-I CD8⁺CD45.1⁺ T cells, injected into recipient C57BL/6 CD45.2⁺ mice. Listeria-WT or Listeria-OVA tpCD4⁺ T cells (2 × 10⁶, a; 5 × 10⁶, b) were adoptively transferred. Proliferation was analyzed 3 days post-transfer. c–h, Analysis of memory CD8⁺ T cell generation in vivo. Rag1^−/− mice were adoptively transferred i.v. with WT CD8⁺ T cells (group 1), Tap1^−/− CD4⁺ T plus WT CD8⁺ T cells (group 2), and WT CD4⁺ T plus WT CD8⁺ T cells (group 3). The following day, all mice were challenged with L. monocytogenes (10³ bacteria/ per mice; i.v.). At 12 (c–e) or 30 days (f–h) after challenge, the CD8⁺ T-cell population from spleen was analyzed. FACS gating is indicating in Supplementary Fig. 4B. c Percentage of CD127⁺, gated on CD3⁺CD8⁺CD44⁺CD62L⁻, pre-memory T cells. Data shown as mean ± s.d. for 3 mice per group (group 1, red; group 2, orange; group 3, blue). *P < 0.05 analyzed by ANOVA and Bonferroni post-test. d Representative dot plots showing CD62L and CD127 staining from the CD3⁺CD8⁺CD44⁺ T-cell population. e Representative histogram showing PD-1 levels on the CD3⁺CD8⁺CD44⁺ T-cell population (group 1, red; group 2, orange; group 3, blue; Fluorescence Minus One (FMO) control, grey). f Representative dot plots showing CD62L and CD44 staining gated on the CD3⁺CD8⁺ T-cell population. The CD62L^high population was also positive for CD127 (not shown). g Percentage of CD62L^highCD44⁺ gated on cells indicated in f (red outline). Data shown as mean ± s.d. for 4 mice per group (group 1, red; group 2, orange; group 3, blue). *P < 0.05 analyzed by ANOVA and Bonferroni post-test. h Representative histogram showing the amount of PD-1 on the CD3⁺CD8⁺CD44⁺ T-cell population (group 1, red; group 2, orange; group 3, blue; FMO control, grey). i L. monocytogenes load in spleen 2 days after a second bacterial challenge (50 days after the first). Each dot represents one mouse (3 mice per group), the mean is shown in red (group 1), orange (group 2) and blue (group 3)