Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 16;8:1552. doi: 10.1038/s41467-017-01584-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Current-time trace and statistics for sensing proteins bound to a modified carrier. Translocation and statistics for a 100 pM of unmodified λ-DNA, b 100 pM aptamer modified λ-DNA (1:1 ratio after filtration), c 100 pM aptamer modified λ-DNA with two probes for the detection of thrombin (1.6 nM each), d 100 pM aptamer modified λ-DNA with three probes for the detection of three thrombin targets (1.6 nM each). All experiments were performed in 100 mM KCl and at a voltage of −200 mV, taken using 4 different nanopipettes for each sample and re-filtered to 5 kHz. Typical individual translocation events are shown with the contribution due to the DNA carrier labelled as “1st step” and the protein contribution labelled as “2nd step”. Scatter plots shown in (iv) clearly indicate binding of protein due to the substantial increase in translocation times and current blockades and corresponding sub-peaks as shown in (iii)