Table 3.
The durations of the individual immobile (a) and mobile (b) events (observed for each single molecule). P values are for comparing PrP and Thy1 (in terms of the duration of each period of the immobile and mobile states) in the same cell type, and show the results of the Mann-Whitney U-test. The differences in the immobile and mobile durations of PrP and Thy1 among the three cell types were complex, and will not be discussed in this report
a. The durations of immobile states | ||||
---|---|---|---|---|
Cells/molecules | Duration of each immobilaiton event (τ obs) (Mean ± SEM [s]) | P for PrP vs. Thy1 | Duration of each immobilation event after correction for photobleaching (τ immob) (Mean ± SEM [s]) | Number of events (n) |
CHO-K1 cells | ||||
PrP | 0.67 ± 0.053 | 0.98 | 1.74 ± 0.36 | 289 |
Thy1 | 0.69 ± 0.090 | 1.84 ± 0.65 | 131 | |
1-wk neurons | ||||
PrP | 0.89 ± 0.076 | 0.56 | 4.69 ± 2.20 | 133 |
Thy1 | 0.87 ± 0.19 | 4.11 ± 4.29 | 31 | |
2-wk neurons | ||||
PrP | 0.79 ± 0.12 | 0.018 | 2.75 ± 1.43 | 67 |
Thy1 | 0.99 ± 0.14 | 10.41 ± 15.02 | 62 |
b. The durations of mobile states | ||||
---|---|---|---|---|
Cells/molecules | Duration of each mobile event (Mean ± SEM [s]) | P for PrP vs. Thy1 | Duration of each mobile event after correction for photobleaching (Mean ± SEM [s]) | Number of events (n) |
CHO-K1 cells | ||||
PrP | 0.45 ± 0.032 | <0.001 | 0.75 ± 0.091 | 381 |
Thy1 | 0.70 ± 0.053 | 1.91 ± 0.41 | 252 | |
1-wk neurons | ||||
PrP | 0.30 ± 0.026 | 0.0064 | 0.41 ± 0.049 | 158 |
Thy1 | 0.46 ± 0.070 | 0.79 ± 0.21 | 59 | |
2-wk neurons | ||||
PrP | 0.28 ± 0.041 | <0.001 | 0.38 ± 0.074 | 70 |
Thy1 | 0.52 ± 0.052 | 0.99 ± 0.19 | 97 |