Fig. 5.

Ras/MAPK pathway mutations in pediatric MDS, MDS/MPN, and AML-MRC. a Heat map showing all Ras/MAPK pathway mutations, both somatic and presumed germline (cells with hatched lines indicate presumed germline variants from WES tumor/normal cases), in the pediatric MDS cohort (n = 77). b Growth curves of Ba/F3 cells transduced with retrovirus containing BRAF mutations. Blue curves indicate mutations found in the pediatric MDS cohort, and black curves are positive (V600E) and negative controls. Error bars indicate standard deviation. c Western blots of BRAF, total ERK, and phosphorylated ERK from lysates of 293 T cells transiently transfected with each BRAF mutation. Data are representative of three biological replicates