Fig. 2.

PfRhopH3 protein is a ligand for Cyclophilin B protein during merozoite binding. a Binding of Cyclophilin B on Merozoite surface. Binding shows binding of CypB on merozoite surface without treatment with any antibody. anti-Rap2b + CypB and anti-RhopH3-C + CypB show binding after treatment with anti-Rap2b and anti-RhopH3 antibody respectively. Intensity for each merozoite is mentioned at the top. Images with histograms represent only the intensity of the bound CypB protein. Anti-RhopH3-C antibody interferes with binding of CypB on merozoites, signifying that PfRhopH3 is a ligand for host Cyclophilin B. Scale bar = 5 µm. b Graph adapted from Supplementary Table 1 showing the average of binding intensity of CypB on 10 different merozoites, with error bars representing the standard deviation. All values were tested for significance using a two-tailed unpaired Student’s t-test. **P < 0.01