Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 16;7:15713. doi: 10.1038/s41598-017-15940-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

PI3K-AKT/TOR signaling in muscles is required for a cellular immune response against wasp infection. (A) Encapsulation rate when affecting insulin signaling, TOR signaling, and Foxo signaling in skeletal muscles with indicated genotypes. (B,C) Number of lamellocytes (B) and plasmatocytes (C) per larva after 12 h wasp infection, when suppressing PI3K signaling or TOR signaling with the indicated genetic constructs. (D,D”) msn-Cherry – labeled lamellocytes in larvae after 12 h wasp infection, when suppressing insulin or TOR signaling with the indicated genetic constructs. (E) Immunostaining of phospho-AKT from larval skeletal muscles, when suppressing insulin signaling by expressing InR ^RNAi or InR ^DN with Mef2-GAL4. Quantification of the immunostained bands is shown in the lower panel. (F) Encapsulation rates when suppressing or activating PI3K signaling in the fat body with the indicated genetic constructs. (G) Encapsulation rate when suppressing or activating PI3K signaling in hemocytes with the indicated genetic constructs. Data information: Encapsulation rates were determined in at least three independent experiments, and in total at least 100 larvae were analyzed. For hemocyte counts, at least eight larvae were analyzed for each genotype. Bars show averages and standard deviations. For immunostaining quantification, at least three independent experiments were analyzed. Bars show the average amounts of phospho-AKT, normalized to total AKT, and standard deviations. The P-values (unpaired t-test, unequal variance) are indicated, NS: not significant.