Table 1.
Crossing of parent stocks to produce the see-through medaka
| Step no. of crossing | Parent stocks
|
Offspring
|
Name of the see-through medaka | ||
|---|---|---|---|---|---|
| Males | Females | Generation | Genotype used for the next step of crossing | ||
| 1 | i-3 (or gu) | gu (or i-3) | F1 | gu/+, i-3/+ | |
| 2 | F1 | F1 | F2 | gu/gu, i-3/i-3 | |
| 3 | F2 | F2 | F3 | gu/gu, i-3/i-3 | STI |
| 4 | lf | STI | F1 | gu/+, i-3/+, Ylf/X+ (Xlf/X+) | |
| 5 | F1 | F1 | F2 | gu/gu, i-3/i-3, Ylf/Xlf | |
| 6 | F2 | F1 | bc* | gu/gu, i-3/i-3, Ylf/Xlf (Xlf/Xlf) | |
| 7 | bc | bc | Intercross | gu/gu, i-3/i-3, Ylf/Xlf (Xlf/Xlf) | STII |
| 8 A | STII | il-1 | F1(A) | gu/+, i-3/+, Ylf/X+ (Xlf/X+), il-1/+ | |
| 8 B | il-1 | STII | F1(B) | gu/+, i-3/+, Y+/Xlf (Xlf/X+), il-1/+ | |
| 9 A | F1(A) | F1(A) | F2(A) | gu/gu, i-3/i-3, Ylf/Xlf, il-1/il-1 | |
| 9 B | F1(B) | F1(B) | F2(B) | gu/gu, i-3/i-3, Xlf/Xlf, il-1/il-1 | |
| 10 | F2(A) | F2(B) | F3 | gu/gu, i-3/i-3, Ylf/Xlf (Xlf/Xlf), il-1/il-1 | STIII |
*
bc, Backcross.