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. 2017 Nov 16;7:15707. doi: 10.1038/s41598-017-15806-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Preparation of surface-modified BDD electrode. (A) Cyclic voltammetry curves recorded for bare and modified BDD electrode in 1 mM K₃Fe(CN)₆/0.1 PBS with a scan rate of 50 mV/s. (B) Impedance spectra of bare and modified BDD electrode after incubation in different solutions recorded in 1 mM K₃Fe(CN)₆/0.1 PBS at E _f = + 0.13 V vs. Ag/AgCl/0.1 M KCl. (C) List of values of elements calculated from the electric equivalent circuit (EEQC) for bare and modified BDD electrode incubated in different solutions. Samples BDD-aM1 – BDD electrode modified with anti-M1 antibody; BDD-aM1-BSA – electrode saturated with BSA; BDD-aM1-PBS – electrode incubated with PBS; BDD-aM1-NEG – electrode incubated with biological samples taken from a healthy man.