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. 2017 Nov 16;7:15752. doi: 10.1038/s41598-017-15793-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Altered expression of NKCC1 and KCC2 in Lgdel ^+/− and Dgcr8 ^+/− neurons. (a) Fluorescence micrographs showing intensity expression of NKCC1 in cultures from WT, Lgdel ^+/− and Dgcr8 ^+/− (n = 5), at 8, 16, 26 DIVs. Scale bar represents 30 μm. (b) Quantification of NKCC1 intensity shows a significantly higher expression in Lgdel ^+/− neurons (n = 5) compared to WT neurons (n = 7) across all developmental ages (*p < 0.05, ANOVA) and slightly higher than Dgcr8 ^+/− neurons (n = 5) (ns denotes not significant). (c) Fluorescence micrographs showing the changes of intensity expression of KCC2 in cultures from the three animal genotypes at 8, 16, 26 DIVs. Scale bar represents 30 μm. (d) Quantification of KCC2 intensity at 8 DIVs shows the non-significant difference between genotypes (ns denotes not significant), but displaying a significantly lower expression in Lgdel ^+/− and Dgcr8 ^+/− neurons compared to WT neurons at 16 and 26 DIVs (*p < 0.05, ANOVA).