Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 16;7:15752. doi: 10.1038/s41598-017-15793-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Neurite outgrowth measurements unveil alteration in neuromorphogenesis in Lgdel ^+/− and Dgcr8 ^+/− neurons. (a) Micrographs showing the raw image of WT neurons (MAP-2) and the segmentation mask for cell bodies (Hoechst) that are used by the neurite outgrowth algorithm to obtain the final cell body and neurite segmentation masks. Scale bar represents 30 µm. (b) Micrographs of WT, Lgdel ^+/− and Dgcr8 ^+/− neurons before segmentation (raw images) and after final segmentation. Scale bar is 30 µm. (c) Quantifications of neurite outgrowth measurements from WT, Lgdel ^+/− and Dgcr8 ^+/− neurons (n = 5 for). Statistical comparisons confirm that all parameters’ values in Lgdel ^+/− and Dgcr8 ^+/− neurons are significantly lower than WT neurons (**p < 0.01, ANOVA). (d) Signal traces of typical spontaneous spikes extracellularly recorded from WT, Lgdel ^+/− and Dgcr8 ^+/− cultures at 8 DIVs. (e) Quantifications of the negative peaks of the spontaneous extracellular spikes recorded from WT, Lgdel ^+/− and Dgcr8 ^+/− cultures (n = 5) at different time points (8, 12, 16, 18 DIVs). This analysis reveals the non-significant difference of the spike amplitudes between the genotypes.