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. 2017 Jun 16;5(4):435–447. doi: 10.1002/iid3.181

Figure 5.

Figure 5

Th1/Th17 cell related cytokine expression in both AE‐DEREG DT‐ and AE‐DEREG DT+ mice at 1 month post‐infection, and in response to ConA. (A) Frequency of IFN‐γ+ within CD4+ T cells in peritoneal and spleen cells from AE‐DEREG DT‐ and AE‐DEREG DT+ mice at 1 month post infection, non‐infected mice as control mice. (B) Representative images of IFN‐γ+ cells within CD4+ T cells in PECs from both AE‐DEREG DT‐ and AE‐DEREG DT+ mice at 1 month post‐infection, non‐infected mice as control mice. (C) Frequency of IL‐17A+ within CD4+ T cells in peritoneal and spleen cells from AE‐DEREG DT‐ and AE‐DEREG DT+ mice at 1 month post infection, non‐infected mice as control mice. (D) Representative images of IL‐17A+ cells within CD4+ T cells in PECs from both AE‐DEREG DT‐ and AE‐DEREG DT+ mice at 1 month post‐infection, non‐infected mice as control mice. (E) Frequency of IFN‐γ+ within CD4+ T cells in peritoneal and spleen cells from AE‐DEREG DT‐ and AE‐DEREG DT+ mice (non‐infected mice as Control mice), co‐cultured with 2 µg/mL ConA. (F) Representative images of IFN‐γ+ cells within CD4+ T cells in spleen cells from both AE‐DEREG DT‐ and AE‐DEREG DT+ mice at 1 month post‐infection (non‐infected mice as Control mice), co‐cultured with 2 µg/mL ConA. (G) Frequency of IL‐17A+ within CD4+ T cells in peritoneal and spleen cells fromAE‐DEREG DT‐ and AE‐DEREG DT+ mice (non‐infected mice as Control mice), co‐cultured with 2 µg/mL ConA. (H) Representative images of IL‐17A+ cells within CD4+ T cells in spleen cells from both AE‐DEREG DT‐ and AE‐DEREG DT+ mice at 1 month post‐infection (non‐infected mice as Control mice), co‐cultured with 2 µg/mL ConA. The same cell reactions performed without ConA were used as non‐stimulated controls. DT application with 110 ng/injection/mouse started 1 day before infection and was maintained for 4 months (three times/week). Data represent mean ± SD of three independent experiments of a total of 8–10 mice in each group (4–5 mice per group in each independent experiment). Comparison between groups was performed using a one‐way ANOVA with Bonferroni's multiple comparison post‐test for statistical analysis. *p < 0.0125. “DEREG DT‐,” foxp3 inducible knock‐down mice (DEREG mice) without DT application; “DEREG DT+,” DEREG mice with DT application; “AE‐ DEREG DT‐,” E. multilocularis‐infected DEREG without DT application; “AE‐ DEREG DT+,” E. multilocularis‐infected DEREG mice with DT application. “Control,” non‐infected mice; “1 m,” 1‐month p.i.; “4 m,” 4 months p.i.. “PEC,” peritoneal exudate cells; “Spleen,” spleen cells.