Figure 2.

DCs from the ocular surface mount allergen-reactive Th2 responses in a CCR7-dependent manner. WT DCs, CCR7^−/− DCs, or no SCJ injection was administered into mice after adoptive transfer with OVA/Alum-primed T cells. After at least 10 days of once daily OVA topical challenges, host T cells were harvested from ipsi LNs and recall stimulated in vitro with OVA. Control wells consisted of T cells from naive mice stimulated with OVA in vitro as well. Supernatant was analyzed via ELISA for IL-4 (A), IL-13 (B), and IL-5 (C). Data are derived from n = 5 hosts per group; figure represents multiple independent experiments. (*P < 0.05)