Figure 3.

Molecular specificity. Samples homozygous for the A*01 allele (A) and the A*02 allele (B) were PCR amplified and NGS sequenced. Each was analyzed for the presence of the other allele and for hybrid molecules containing one or more SNPs of the other allele. C: Waterfall plot of the percentage reads for each combination of the 11 SNP differences for the two samples. For example, the 10A1 + 1A2 bar reflects the mean number of erroneous reads (9/11 = 0.82), in the pure A*01 sample where any one of the 11 SNP positions matches the A*02 allele (0.82/201,605, from A). Likewise, the 9A1 + 2A2 bar reflects the lack of any reads in the pure A*01 sample, where two adjacent SNPs match the A*02 allele at any of the positions.