Figure 5.

PTEN is a direct target gene of miR-20b in prostate cancer cells. (A) Presentation of the miR-20b binding region within the mRNA 3′-untranslated region of PTEN. (B) VCaP and PC-3 cells were co-transfected with psiCHECK-2-PTEN-WT or psiCHECK-2-PTEN-MUT and miR-20b inhibitor or inhibitor control for 48 h, and the luciferase activities were assessed to determine the direct interaction between miR-20b and PTEN. *P<0.05. (C) Western blot analysis detected PTEN expression in VCaP and PC-3 cells transfected with miR-20b inhibitor or inhibitor control. GAPDH was used as an internal control. PTEN, phosphatase and tensin homolog; WT, wild-type; MUT, mutant; miR-20b, microRNA-20b.