Figure 2.

HMGN5 serves as a target for CDDP treatment in UBC cells. Different concentrations (0, 1, 2, 8 and 16 µg/ml) of CDDP were used to treat UBC 5637 cells for 24 h. The expression of HMGN5 and its downstream signal molecules, including Akt, p-Akt, slug, E-cadherin and VEGF-C, was then analyzed by western blot analysis. The expression of a number of markers in the mitochondrial apoptosis pathway, including cytochrome c, cleaved-caspase-3 and cleaved-PARP, was also analyzed. β-actin served as a loading control. HMGN5, high mobility group nucleosome-binding domain 5; CDDP, cisplatin; p-, phosphorylated; PARP, poly[ADP-ribose] synthase 1; VEGF, vascular endothelial growth factor.