Figure 3.

HMGN5 depletion impairs the viability and clonogenicity in UBC cells. (A) Dose-dependent MTT assay was employed to investigate the effect of HMGN5-knockdown on viability of 5637 cells and the effect of IGF-1 (a PI3K signaling activator) on the viability of 5637 cells with HMGN5-knockdown following CDDP treatment for 24 h. (B) Colony formation assay was utilized to detect the effect of HMGN5-knockdown on the clonogenicity in 5637 cells and the effect of IGF-1 on the clonogenicity in 5637 cells with HMGN5 inhibition. (C) Quantitative summary of colony formation in different groups. Results are expressed as the mean ± standard deviation (n=3), **P<0.01 compared with HMGN5-knocked down 5637 cells. CDDP, cisplatin; HMGN5, high mobility group nucleosome-binding domain 5; IGF-1, insulin-likegrowth factor-1; PI3K, phosphoinositide 3-kinase; sh, short hairpin; Con, control; IC₅₀, Half-maximal inhibitory concentration.