TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| C. pinatubonensis JMP134 | Wild type | Ron L. Crawford |
| E. coli BL21(DE3) | F−ompT hsdSB (rB− mB−) gal dcm met (DE3) | Invitrogen |
| Plasmids | ||
| pET30 Ec/Lic | Kmr, expression vector | Invitrogen |
| pBBR1MCS2 | Kmr, expression vector | Invitrogen |
| pET30-Cpsqr | pET30 Ec/Lic containing Cpsqr gene from C. pinatubonensis JMP134 | This study |
| pET30-Cpduf442 | pET30 Ec/Lic containing Cpduf442 gene from C. pinatubonensis JMP134 | 40 |
| pBBR2-signal-phoA | pBBR1MCS2 containing phoA encoding the full-length PhoA with its signal peptide from E. coli BL21(DE3) | This study |
| pBBR2-phoA | pBBR1MCS2 containing a phoA gene fragment that encodes PhoA without its signal peptide | This study |
| pBBR2-gapA-gfp | pBBR1MCS2 containing gapA of E. coli MG1655 fused with the GFP gene | This study |
| pBBR2-mhpT-gfp | pBBR1MCS2 containing mhpT (41) of E. coli MG1655 fused with the GFP gene | This study |
| pBBR2-tolB-phoA | pBBR1MCS2 containing tolB (14) of E. coli MG1655 fused with phoA | This study |
| pBBR2-Cpsqr-gfp | pBBR1MCS2 containing Cpsqr fused with the GFP gene | This study |
| pBBR2-Cpsqr-phoA | pBBR1MCS2 containing Cpsqr fused with phoA | This study |
| pBBR2-fragmentx-gfp | pBBR1MCS2 containing a Cpsqr fragment encoding the N-terminal x amino acid residues fused with the GFP gene | This study |
| pBBR2-fragmentx-phoA | pBBR1MCS2 containing a Cpsqr fragment encoding the N-terminal x amino acid residues fused with phoA | This study |
| pBBR2-Cppdo2-phoA | pBBR1MCS2 containing Cppdo2 fused with phoA | This study |
| pBBR2-Cppdo2-gfp | pBBR1MCS2 containing Cppdo2 fused with the GFP gene | This study |
| pBBR2-Cppdo1-gfp | pBBR1MCS2 containing Cppdo1 fused with the GFP gene | This study |
| pBBR2-Cppdo1-phoA | pBBR1MCS2 containing Cppdo1 fused with phoA | This study |
| pBBR2-Papdo2-gfp | pBBR1MCS2 containing Papdo2 (21) of Pseudomonas aeruginosa PAO1 fused with the GFP gene | This study |
| pBBR2-Papdo2-phoA | pBBR1MCS2 containing Papdo2 fused with phoA | This study |
| pBBR2-Mxpdo1a-gfp | pBBR1MCS2 containing Mxpdo1a (21) of Myxococcus xanthus DK 1622 fused with the GFP gene | This study |
| pBBR2-Mxpdo1a-phoA | pBBR1MCS2 containing Mxpdo1a fused with phoA | This study |
| pBBR2-Atblh-gfp | pBBR1MCS2 containing Atblh (21) of Agrobacterium tumefaciens C58 fused with the GFP gene | This study |
| pBBR2-Atblh-phoA | pBBR1MCS2 containing Atblh fused with phoA | This study |
| pBBR2-EcgloB-gfp | pBBR1MCS2 containing EcgloB (21) of E. coli MG1655 fused with the GFP gene | This study |
| pBBR2-EcgloB-phoA | pBBR1MCS2 containing EcgloB fused with phoA | This study |