TABLE 3.
Oligonucleotide primers used for plasmid construction
| Primer | Nucleotide sequencea | Target protein |
|---|---|---|
| gfp-F | CACACAGGAAACAGCTATGAGTAAAGGAGAAGAAC | GFP |
| gfp-R | TAACAAAATATTAACGCTTATTTGTATAGTTCATACAT | |
| phoA-F | CACACAGGAAACAGCTATGCGGGCACCAGAAATGC | PhoA |
| phoA-R | TATTTGTACAAAATATTAACGCTTATTTCAGCCCCAGAGCG | |
| Signal-phoA-F | CACACAGGAAACAGCTATGAAACAAAGCACTATTGCAC | Signal-PhoA |
| Cpsqr-F | CACACAGGAAACAGCTATGCAACCCCGCGCCCTCACC | CpSQR |
| Cpsqr-R | AACAAAATATTAACGCTTATCCCTGCAACTCGGGT | |
| Cpsqr-phoA-R | TCCTGCTGCACTTCCTGCACTTCCTCCCTGCAACTCGGGTG | CpSQR-linker |
| Cpsqr-phoA-F | CAGCAGGAAGTGGAGAATTCCGGGCACCAGAAATGCCTG | Linker-PhoA |
| Cpsqr-gfp-F | CAGCAGGAAGTGGAGAATTCATGAGTAAAGGAGAAGAACT | Linker-GFP |
| Cpduf-gfp-R | TGCTGCACTTCCTGCACTTCCTAGATCATAGCCGGCGGCC | CpDUF442-linker |
| Linker-F | GGAAGTGCAGGAAGTGCAGCAGGAAGTG | Linker-PhoA/GFP |
| Fragment26-phoA-R | TTCCTGCTGCACTTCCTGCACTTCCGGCCGCCAGAGCGTGC | FragmentR6-linker |
| Fragment49-phoA-R | TCCTGCTGCACTTCCTGCACTTCCTTCCTGGAAGTTTGGCT | Fragment49-linker |
| Fragment71-phoA-R | TTCCTGCTGCACTTCCTGCACTTCCGACCTTGCCCGATTCC | Fragment71-linker |
| Fragment83-phoA-R | CCTGCTGCACTTCCTGCACTTCCCAGGAGTTGGCCGAACG | Fragment83-linker |
| Fragment106-phoA-R | TTCCTGCTGCACTTCCTGCACTTCCCAGCGCCCACAGCGTC | Fragment106-linker |
| Fragment115-phoA-R | CCTGCTGCACTTCCTGCACTTCCCGGCAGTGGCCTGATCAC | Fragment115-linker |
| Fragment157-phoA-R | TTCCTGCTGCACTTCCTGCACTTCCGCCGCCAATGATGACC | Fragment157-linker |
| Fragment176-phoA-R | CTTCCTGCTGCACTTCCTGCACTTCCATCCAGCCCCGGC | Fragment176-linker |
| Fragment230-phoA-R | CTTCCTGCTGCACTTCCTGCACTTCCCTCGGGCTCGAAGG | Fragment230-linker |
| Fragment359-phoA-R | CTTCCTGCTGCACTTCCTGCACTTCCGACGTACTGCATCA | Fragment359-linker |
| Fragment450-phoA-R | CTTCCTGCTGCACTTCCTGCACTTCCGTTGCCGGCGTCGC | Fragment450-linker |
| tolB-phoA-F | CACACAGGAAACAGCTATGAAGCAGGCATTACGAG | TolB-linker |
| tolB-phoA-R | CCTGCTGCACTTCCTGCACTTCCCAGATACGGCGACCAGG | |
| mhpT-gfp-F | CACACAGGAAACAGCTATGTCGACTCGTACCCCTT | MhpT-linker |
| mhpT-gfp-R | TGCTGCACTTCCTGCACTTCCGGCATCGGCGCACGGCTGTA | |
| Cppdo1-gfp-F | ACACAGGAAACAGCTATGCAAACCTTCTAT | CpPDO1-linker |
| Cppdo1-gfp-R | ACTTCCTGCACTTCCGGCGCCATGCGGCACG | |
| Cppdo2-gfp-F | CACACAGGAAACAGCTATGACACCGACCATGCCAAG | CpPDO2-linker |
| Cppdo2-gfp-R | TGCTGCACTTCCTGCACTTCCGAGGGCGTTGAGGGGAATCT | |
| Papdo2-gfp-F | ACACAGGAAACAGCTATGTTGAAACCCGAC | PaPDO2-linker |
| Papdo2-gfp-R | TGCACTTCCTGCACTTCCGAACAGATCCAGCGGGA | |
| Atblh-gfp-F | ACACAGGAAACAGCTATGAAGGCCGTAAGG | AtBlh-linker |
| Atblh-gfp-R | ACTTCCTGCACTTCCCCATGTGCTGCCCTCCAG | |
| Mxpdo1a-gfp-F | ACACAGGAAACAGCTATGCTCTTCCGCCAG | MxPDO1a-linker |
| Mxpdo1a-gfp-R | ACTTCCTGCACTTCCATGTGTGAAGCTGCCTC | |
| gloB-gfp-F | ACACAGGAAACAGCTATGAATCTTAACAGT | GloB-linker |
| gloB-gfp-R | ACTTCCTGCACTTCCGAACCTATCTTTCTTTG | |
| gapA-gfp-F | CACACAGGAAACAGCTATGACTATCAAAGTAGGTAT | GAPDH-linker |
| gapA-gfp-R | CACTTCCTGCACTTCCTTTGGAGATGTGAGCGAT | |
| (pET)Cpsqr-F | TAAGAAGGAGATATACATATGCAACCCCGCGCCCT | CpSQR |
| (pET)Cpsqr-R | GTGGTGGTGCTCGAGTCCCTGCAACTCGGGTGT |
a
Underlining indicates sequences overlapping with the linker.