FIG 2.

Maps of the regions containing F. columnare strain C#2 gldN (A) and porV (B) and associated deletions. Numbers below the maps refer to kbp of sequence. Binding sites for primers used in PCRs to generate deletion or complementation constructs are shown above and below the maps, with the blunt ends indicating the actual binding sites. The horizontal lines beneath the maps marked with open triangles denote regions deleted from the chromosome in the mutants. The regions of DNA carried by complementation plasmids pLN8 and pLN11 are indicated beneath the maps. The map of the gldN region of F. columnare strain IA-S-4 is identical to that shown in panel A, and F. columnare IA-S-4 gldN deletion and complementation constructs were constructed in the same way using the primers and plasmids listed in Tables 3 and 2, respectively.