FIG 5.
Digestion of proteins and chondroitin sulfate by material secreted by wild-type and mutant cells. Cells examined were wild-type F. columnare IA-S-4, ΔgldN mutant in strain IA-S-4 (ΔgldNIA-S-4), ΔgldNIA-S-4 mutant complemented with plasmid pLN5, wild-type F. columnare C#2, ΔgldN mutant in strain C#2 (ΔgldNC#2), ΔgldNC#2 mutant complemented with plasmid pLN8, ΔporV mutant in strain C#2 (ΔporVC#2), and ΔporVC#2 mutant complemented with plasmid pLN11. Digestion of protein (azocasein) (A) or of chondroitin sulfate (B) by cell-free spent medium from each strain was determined as described in Materials and Methods. The values are the means and the error bars indicate standard deviations (n = 3).