TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Growth medium | Reference or source |
|---|---|---|---|
| Plasmids | |||
| pSRKKm-Plac-sfgfp | Kmr, broad host range vector containing lacIq and lac promoter | 63 | |
| pSRKKm-Plac-PPH | PPHa inserted into pSRKKm-Plac-sfgfp | This study | |
| pSRKKm-Plac-PPHE32A | PPH predicted catalytic residue mutated | This study | |
| pSRKKm-Plac-PPHK328A,K334A,K335A | PPH regulatory residues mutated | This study | |
| pSRKKm-Plac-PPHE32A,K328A,K334A,K335A | PPH catalytic and regulatory residues mutated | This study | |
| pSRKKm-Plac-PPH1–304 | PPH truncation to remove TM domain | This study | |
| E. coli strains | |||
| DH5α | Cloning strain, gammaproteobacterium | LB | Life Technologies |
| S17-1 | Smr, RP4-2, Tc::Mu, Km-Tn7, for plasmid mobilization | LB | 83 |
| A. tumefaciens strains | |||
| C58 | Nopaline type strain; pTiC58; pAtC58 | LB | 32 |
| C58(pSRKKm-Plac-sfgfp) | C58 transformed with empty pSRKKm plasmid | LB | 63 |
| C58(pSRKKm-Plac-PPH) | C58 transformed with pSRKKM-Plac-PPH | LB | This study |
| C58(pSRKKm-Plac-PPHE32A) | C58 transformed with pSRKKm-Plac-PPHE32A | LB | This study |
| C58(pSRKKm-Plac-PPHK328A,K334A,K335A) | C58 transformed with pSRKKm-Plac-PPHK328A,K334A,K335A | LB | This study |
| C58(pSRKKm-Plac-PPHE32A,K328A,K334A,K335A) | C58 transformed with pSRKKm-Plac-PPHE32A,K328A,K334A,K335A | LB | This study |
| C58(pSRKKm-Plac-PPH1–304) | C58 transformed with pSRKKm-Plac-PPH1–304 | LB | This study |
| EHA105 | C58 derived, succinamopine strain, T-DNA deletion derivative of pTiBo542 | LB | MU plant transformation core facility |
| EHA101 | C58 derived, nopaline strain, T-DNA deletion derivative of pTiBo542 | LB | MU plant transformation core facility |
| GV3101 | C58 derived, nopaline strain | LB | MU plant transformation core facility |
| NTL4 | C58 derived, nopaline-agrocinopine strain, ΔtetRA | LB | 84 |
| AGL-1 | C58 derived, succinamopine strain, T-DNA deletion derivative of pTiBo542 ΔrecA | LB | MU plant transformation core facility |
| LBA4404 | Ach5 derived, octopine strain, T-DNA deletion derivative of pTiAch5 | YM | MU plant transformation core facility |
| Chry5 | Succinamopine strain, pTiChry5 | LB | 85 |
| Other bacterial strains | |||
| Agrobacterium vitis S4 | Vitopine strain, pTiS4, pSymA, pSymB | Potato dextrose | 86 |
| Sinorhizobium meliloti RM41 | Rhizopine strain, pSymA, pSymB, pRme41a | LB | 87 |
| Caulobacter crescentus CB15 | Alphaproteobacterium | PYE | 88 |
a
PPH, phage peptidoglycan hydrolase.