TABLE 4.
Primers for 81-176 poly(G)-containing genes
| Gene | NCTC11168 homologa | Primer | Primer sequence |
|---|---|---|---|
| CJJ81176_0082 | Cj0044c | 81176-0082-fwd | GGTTTTACACTAGAACACAGAAG |
| 81176-0082-fwd-FAM | FAM-GGTTTTACACTAGAACACAGAAG | ||
| 81176-0082-rev | CCATACACGAAGAACTTGTTAGCAA | ||
| CJJ81176_0086 | NA | 81176-0086-fwd | GCACAAGCTACAAATTTAGAAGTG |
| 81176-0086-fwd-FAM | FAM-GCACAAGCTACAAATTTAGAAGTG | ||
| 81176-0086-rev | AAGTAGAAGCATTAGGCGTGGAT | ||
| CJJ81176_0206 | Cj0171 | 81176-0206-fwd | TGTTGTGGAAATGGAGTGCATTC |
| 81176-0206-fwd-NED | TGGTTGTGGAAATGGAGTGCATTC | ||
| 81176-0206-rev | AATAGCTCCTTCATTGCATAGTTC | ||
| CJJ81176_0590 | Cj0565 | 81176-0590-fwd | GCTATAGTGTTGATGTGTAGTTTGT |
| 81176-0590-fwd-FAM | FAM-GCTATAGTGTTGATGTGTAGTTTGT | ||
| 81176-0590-rev | CCTTTATCAATTTCTATTCTTAGAC | ||
| CJJ81176_0646 | Cj0617 | 81176-0646-fwd | TGGTATAATGCAAGCTATGG |
| 81176-0646-fwd-VIC | TGGTATAATGCAAGCTATGG | ||
| 81176-0646-rev | AAATCAATACTCCAAGGAGC | ||
| CJJ81176_0708 | Cj0685 | 81176-0708-fwd | GATAGCGAATATAACCTCTAAATTC |
| 81176-0708-fwd-FAM | FAM-GATAGCGAATATAACCTCTAAATTC | ||
| 81176-0708-rev | GAAGAAATCCGCCAATCAAAGGCC | ||
| CJJ81176_0758 | Cj0735 | 81176-0758-fwd | GCAAACCAAGCATAGGGGATTGTGG |
| 81176-0758-fwd-FAM | FAM-GCAAACCAAGCATAGGGGATTGTGG | ||
| 81176-0758-rev | TTCACCATCAAAAGCTACAGATCCTG | ||
| CJJ81176_0765 | NA | 81176-0765-fwd | TGCAAGAGCTGTTCATCGGTTTAAAC |
| 81176-0765-fwd-NED | TGCAAGAGCTGTTCATCGGTTTAAAC | ||
| 81176-0765-rev | CTTAAAAGGGGATTAAATAAAGGAT | ||
| CJJ81176_1160 | Cj1143 | 81176-1160-fwd | CGATGCCAAGAAGCTTTATAAGAGTT |
| 81176-1160-fwd-FAM | FAM-CGATGCCAAGAAGCTTTATAAGAGTT | ||
| 81176-1160-rev | GCTTGTAGTATTTTCACTCCTTGAGAT | ||
| CJJ81176_1312 | Cj1295 | 81176-1312-fwd | TTCCTATCCCTAGGAGTATC |
| 81176-1312-fwd-NED | TTCCTATCCCTAGGAGTATC | ||
| 81176-1312-rev | ATAGGCTTCTTTAGCGTTCC | ||
| CJJ81176_1321 | Cj1305c | 81176-1321-27-fwd | CAACTTTTATCCCACCTAATGGAG |
| 81176-1321-27-fwd-VIC | CAACTTTTATCCCACCTAATGGAG | ||
| 81176-1321-rev | GTGGAAAATCTTCATAATATTCACTG | ||
| CJJ81176_1325 | NA | 81176-1325-fwd | GCTTGATGATAATTTTACCGCCTTAAG |
| 81176-1325-rev | CAATCTAGCCTTAGGCACTCCGATT | ||
| CJJ81176_1327 | NA | 81176-1325-fwd-FAM | FAM-GCTTGATGATAATTTTACCGCCTTAAG |
| 81176-1327-rev | TCTCAATAACGGCAAAACAGTTCCC | ||
| CJJ81176_1341 | Cj1342 | 81176-1341-fwd | GGTAATCGTCCTCAAACAGG |
| 81176-1341-fwd-FAM | FAM-GGTAATCGTCCTCAAACAGG | ||
| 81176-1341-rev | GCCAAATGCGCTAAATATCC | ||
| CJJ81176_1419 | Cj1420c | 81176-1419-fwd | GCTAGTTCTTTCCATTGGAC |
| 81176-1419-fwd-FAM | FAM-GCTAGTTCTTTCCATTGGAC | ||
| 81176-1419-rev | TGCCACTGCTTACACGAGCAAG | ||
| CJJ81176_1421 | Cj1422c | 81176-1421-35-rev | TTGAGCTGAGGAAAGATTTGTATAG |
| 81176-1421-fwd | TATTGGTGTGCCTGAGGATTATTAT | ||
| 81176-1421-fwd-VIC | TATTGGTGTGCCTGAGGATTATTAT | ||
| CJJ81176_1429 | Cj1429c | 81176-1429-fwd | CTCCTATTCTTTCAGAACGTGATAT |
| 81176-1429-fwd-FAM | FAM-CTCCTATTCTTTCAGAACGTGATAT | ||
| 81176-1429-rev | CTATGCTAGCATCATATTCAATTACC | ||
| CJJ81176_1432 | Cj1138 | 81176-1432-fwd | CTAGGACAGGCTTTGATTATAAATTC |
| 81176-1432-fwd-VIC | CTAGGACAGGCTTTGATTATAAATTC | ||
| 81176-1432-rev | CACCATCATCTCTAACAGATATAAAT | ||
| CJJ81176_1435 | NA | 81176-1435-fwd | ATCTTAATACCTGATTTTATCAAAAC |
| 81176-1435-fwd-NED | ATCTTAATACCTGATTTTATCAAAAC |
a
NA, not applicable (no homolog in NCTC11168).