TABLE 3.
Strains and vectors used in this study
| Straina or vector | Relevant characteristic(s)b | Source or reference |
|---|---|---|
| Strains | ||
| Acidovorax sp. T1 (= CCTCC M2016205) | Degrades thiobencarb | This study |
| Acidovorax sp. T1m | Mutant of T1; degrades 4CBA and 4CDA, but not thiobencarb | This study |
| E. coli DH5α | Host strain for cloning vector | TaKaRa |
| E. coli BL21(DE3) | Host strain for expression vector | TaKaRa |
| Vectors | ||
| pMD19-T | TA clone vector; Ampr | TaKaRa |
| pBBR-tmoA | pBBR1MCS-2 derivative carrying tmoA; Kmr | This study |
| pBBR-tmoAB | pBBR1MCS-2 derivative carrying tmoAB; Kmr | This study |
| pBBR-tmoB | pBBR1MCS-2 derivative carrying tmoB; Kmr | This study |
| pET24b(+) | Expression vector; Kmr | Novagen |
| pET-tmoA | pET24b(+) derivative carrying tmoA; Kmr | This study |
| pET-tmoB | pET24b(+) derivative carrying tmoB; Kmr | This study |
| pET-tmoC | pET24b(+) derivative carrying tmoC; Kmr | This study |
a
CCTCC, China Center for Type Culture Collection.
b
Kmr, kanamycin resistant; Ampr, ampicillin resistant.