Functional validation of L. crescens gloA in the heterologous host X. albilineans. (A) X. albilineans cells carrying L. crescens
gloA (pMJ052), X. albilineans gloA (pMJ054), or empty vector (pUFR071) were grown for 4 days in modified Wilbrink's medium supplemented with 0 to 25 mM methylglyoxal. (B) Extractable glyoxalase I enzyme activity (S-lactoylglutathione, min−1 mg of protein−1) in X. albilineans cells carrying L. crescens gloA (pMJ052), X. albilineans gloA (pMJ054), or empty vector (pUFR071). The bars represent averages ± the standard errors (A, n = 8; B, n = 4). Asterisks represent significant differences at P < 0.05.