FIG 3.

Functional characterization of L. crescens sucrose transporter sut (B488_RS00965). Five-day-old wild-type BT-1 and interruption mutant Sut-1 cells (sut::pCR2.1-TOPO) were incubated in the presence of [¹⁴C]sucrose at room temperature. At the indicated time points, 500-μl culture aliquots were withdrawn, washed twice to remove external [¹⁴C]sucrose, and resuspended in PBS and scintillation fluid. The amount of radioactive sucrose taken up by the cells was expressed as counts per minute (cpm). Bars represent average data ± standard errors of at least three independent experiments.